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f 10 nutrient mix gibco thermo fisher gibco thermo fisher  (Thermo Fisher)


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    Thermo Fisher f 10 nutrient mix gibco thermo fisher gibco thermo fisher
    F 10 Nutrient Mix Gibco Thermo Fisher Gibco Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+10+nutrient+mix+gibco+thermo+fisher+gibco+thermo+fisher/Dynabeads+Protein+G+for+Immunoprecipitation/10__7554_slash_elife__31023-218-94-97
    Average 99 stars, based on 12245 article reviews
    f 10 nutrient mix gibco thermo fisher gibco thermo fisher - by Bioz Stars, 2026-09
    99/100 stars

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    Modification:

    Article Title: NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins
    Article Snippet: DOI: https://doi.org/10.7554/eLife.31023 15 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody TRITC anti-rabbit Jackson ImmunoResearch Jackson Immuno Research:711025152; RRID:AB_2340588 Recombinant DNA reagent pCW57.1-MBD3L2 This paper Addgene plasmid #106332 Lentiviral vector expressing doxycycline-inducible MBD3L2 (generated using pCW57.1 [Addgene plasmid #41393]) Recombinant DNA reagent pGIPZ-shControl Fred Hutchinson Cancer Research Center Genomics Shared Resource (http://monod. fhcrc.org/rnai/) Recombinant DNA reagent pGIPZ-shMBD3L-1 Fred Hutchinson Cancer Research Center Genomics Shared Resource (http://monod. fhcrc.org/rnai/) Recombinant DNA reagent pGIPZ-shMBD3L-2 Fred Hutchinson Cancer Research Center Genomics Shared Resource (http://monod. fhcrc.org/rnai/) Recombinant DNA reagent pZLCv2-3xFLAG-dCas 9-HA-2xNLS This paper Addgene plasmid #106357 Lentiviral vector expressing FLAGtagged, nuclease-deficient Cas9 (generated using lentiCRISPRv2 [Addgene plasmid #52961] and pHR-SFFV-KRAB-dCas9-P2AmCherry [Addgene plasmid #60954]) Recombinant DNA reagent pZLCv2-gD4Z4-1-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106352 Lentiviral vector expressing FLAGdCas9 and a guide RNA targeting the D4Z4 unit Recombinant DNA reagent pZLCv2-gD4Z4-2-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106353 Lentiviral vector expressing FLAGdCas9 and a gRNA targeting the D4Z4 unit Recombinant DNA reagent pZLCv2-gD4Z4-3-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106354 Lentiviral vector expressing FLAGdCas9 and a gRNA targeting the D4Z4 unit Recombinant DNA reagent pZLCv2-gMYOD1-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106355 Lentiviral vector expressing FLAGdCas9 and a gRNA targeting the MYOD1 distal regulatory region Sequenced-based reagent enChIP-/ChIPqPCR primers This paper See Supplementary file 4 Sequenced-based reagent gRNAs This paper See Supplementary file 4 Sequenced-based reagent RT-qPCR primers This paper See Supplementary file 4 Sequenced-based reagent shRNAs This paper See Supplementary file 4 Sequenced-based reagent siRNAs This paper See Supplementary file 4 Peptide, recombinant protein 3X FLAG peptide Sigma-Aldrich Sigma-Aldrich:F4799 Commercial assay or kit QIAshredder Qiagen Qiagen:79656 Commercial assay or kit RNeasy Mini Kit Qiagen Qiagen:74106 Continued on next page Campbell et al. eLife 2018;7:e31023. .. DOI: https://doi.org/10.7554/eLife.31023 16 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit SuperScript III First-Strand Synthesis System Invitrogen/Thermo Fisher Invitrogen/Thermo Fisher:18080051 Chemical compound, drug 2-Mercaptoethanol Sigma-Aldrich Sigma-Aldrich:M3148 Chemical compound, drug Dexamethasone Sigma-Aldrich Sigma-Aldrich:D4902 Chemical compound, drug DMEM:Nutrient Mixture F-12 Gibco/Thermo Fisher Gibco/Thermo Fisher:11320082 Chemical compound, drug DNase I Thermo Fisher Thermo Fisher:18068015 Chemical compound, drug Doxycyline hyclate Sigma-Aldrich Sigma-Aldrich:D9891 Chemical compound, drug Dulbecco’s Modified Eagle Medium (DMEM) Gibco/Thermo Fisher Gibco/Thermo Fisher:11965092 Chemical compound, drug Dynabeads-Protein G Thermo Fisher Thermo Fisher:10003D Chemical compound, drug Ham’s F-10 Nutrient Mix Gibco/Thermo Fisher Gibco/Thermo Fisher:11550043 Chemical compound, drug Horse serum Gibco/Thermo Fisher Gibco/Thermo Fisher:26050070 Chemical compound, drug HyClone Fetal Bovine Serum GE Healthcare Life Sciences GE Healthcare Life Sciences:SH30071.03 Chemical compound, drug Insulin Sigma-Aldrich Sigma-Aldrich:I1882 Chemical compound, drug KnockOut Serum Replacement Gibco/Thermo Fisher Gibco/Thermo Fisher:10828028 Chemical compound, drug Lipofectamine RNAiMAX Invitrogen/Thermo Fisher Invitrogen/Thermo Fisher:13778150 Chemical compound, drug Matrigel Corning Life Science Corning Life Science:354277 Chemical compound, drug MEM non-essential amino acids Gibco/Thermo Fisher Gibco/Thermo Fisher:11140050 Chemical compound, drug mTeSR1 medium STEMCELL Technologies STEMCELL Technologies:85850 Chemical compound, drug Opti-MEM reduced serum medium Thermo Fisher Thermo Fisher:31985070 Chemical compound, drug Penicillin/streptomycin Gibco/Thermo Fisher Gibco/Thermo Fisher:15140122 Chemical compound, drug Polybrene Sigma-Aldrich Sigma-Aldrich:107689 Chemical compound, drug Puromycin Sigma-Aldrich Sigma-Aldrich:P8833 Chemical compound, drug Recominant human basic fibroblast growth factor Promega Corporation Promega Corporation: G5071 Chemical compound, drug Sodium pyruvate Gibco/Thermo Fisher Gibco/Thermo Fisher:11360070 Chemical compound, drug Transferrin Sigma-Aldrich Sigma-Aldrich:T0665 Chemical compound, drug Y-27632 ROCK inhibitor Miltenyi Biotec Miltenyi Biotec: 130106538 Continued on next page Campbell et al. eLife 2018;7:e31023. .. DOI: https://doi.org/10.7554/eLife.31023 17 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Code used for proteomics data analysis This paper (Jagannathan, 2017) The R code used for the proteomics data analysis can be accessed via github at https://github.com/sjaganna/ 2017-campbell_et_al Software, algorithm GraphPad Prism GraphPad Prism (https://graphpad.com) RRID:SCR_015807 Version 6 Software, algorithm ImageJ ImageJ (http://imagej.nih.gov/ij/) RRID:SCR_003070 Software, algorithm Proteome Discoverer Thermo Fisher RRID:SCR_014477 Version 1.4

    Knock-Out:

    Article Title: NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins
    Article Snippet: DOI: https://doi.org/10.7554/eLife.31023 15 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody TRITC anti-rabbit Jackson ImmunoResearch Jackson Immuno Research:711025152; RRID:AB_2340588 Recombinant DNA reagent pCW57.1-MBD3L2 This paper Addgene plasmid #106332 Lentiviral vector expressing doxycycline-inducible MBD3L2 (generated using pCW57.1 [Addgene plasmid #41393]) Recombinant DNA reagent pGIPZ-shControl Fred Hutchinson Cancer Research Center Genomics Shared Resource (http://monod. fhcrc.org/rnai/) Recombinant DNA reagent pGIPZ-shMBD3L-1 Fred Hutchinson Cancer Research Center Genomics Shared Resource (http://monod. fhcrc.org/rnai/) Recombinant DNA reagent pGIPZ-shMBD3L-2 Fred Hutchinson Cancer Research Center Genomics Shared Resource (http://monod. fhcrc.org/rnai/) Recombinant DNA reagent pZLCv2-3xFLAG-dCas 9-HA-2xNLS This paper Addgene plasmid #106357 Lentiviral vector expressing FLAGtagged, nuclease-deficient Cas9 (generated using lentiCRISPRv2 [Addgene plasmid #52961] and pHR-SFFV-KRAB-dCas9-P2AmCherry [Addgene plasmid #60954]) Recombinant DNA reagent pZLCv2-gD4Z4-1-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106352 Lentiviral vector expressing FLAGdCas9 and a guide RNA targeting the D4Z4 unit Recombinant DNA reagent pZLCv2-gD4Z4-2-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106353 Lentiviral vector expressing FLAGdCas9 and a gRNA targeting the D4Z4 unit Recombinant DNA reagent pZLCv2-gD4Z4-3-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106354 Lentiviral vector expressing FLAGdCas9 and a gRNA targeting the D4Z4 unit Recombinant DNA reagent pZLCv2-gMYOD1-3x FLAG-dCas9-HA-2xNLS This paper Addgene plasmid #106355 Lentiviral vector expressing FLAGdCas9 and a gRNA targeting the MYOD1 distal regulatory region Sequenced-based reagent enChIP-/ChIPqPCR primers This paper See Supplementary file 4 Sequenced-based reagent gRNAs This paper See Supplementary file 4 Sequenced-based reagent RT-qPCR primers This paper See Supplementary file 4 Sequenced-based reagent shRNAs This paper See Supplementary file 4 Sequenced-based reagent siRNAs This paper See Supplementary file 4 Peptide, recombinant protein 3X FLAG peptide Sigma-Aldrich Sigma-Aldrich:F4799 Commercial assay or kit QIAshredder Qiagen Qiagen:79656 Commercial assay or kit RNeasy Mini Kit Qiagen Qiagen:74106 Continued on next page Campbell et al. eLife 2018;7:e31023. .. DOI: https://doi.org/10.7554/eLife.31023 16 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit SuperScript III First-Strand Synthesis System Invitrogen/Thermo Fisher Invitrogen/Thermo Fisher:18080051 Chemical compound, drug 2-Mercaptoethanol Sigma-Aldrich Sigma-Aldrich:M3148 Chemical compound, drug Dexamethasone Sigma-Aldrich Sigma-Aldrich:D4902 Chemical compound, drug DMEM:Nutrient Mixture F-12 Gibco/Thermo Fisher Gibco/Thermo Fisher:11320082 Chemical compound, drug DNase I Thermo Fisher Thermo Fisher:18068015 Chemical compound, drug Doxycyline hyclate Sigma-Aldrich Sigma-Aldrich:D9891 Chemical compound, drug Dulbecco’s Modified Eagle Medium (DMEM) Gibco/Thermo Fisher Gibco/Thermo Fisher:11965092 Chemical compound, drug Dynabeads-Protein G Thermo Fisher Thermo Fisher:10003D Chemical compound, drug Ham’s F-10 Nutrient Mix Gibco/Thermo Fisher Gibco/Thermo Fisher:11550043 Chemical compound, drug Horse serum Gibco/Thermo Fisher Gibco/Thermo Fisher:26050070 Chemical compound, drug HyClone Fetal Bovine Serum GE Healthcare Life Sciences GE Healthcare Life Sciences:SH30071.03 Chemical compound, drug Insulin Sigma-Aldrich Sigma-Aldrich:I1882 Chemical compound, drug KnockOut Serum Replacement Gibco/Thermo Fisher Gibco/Thermo Fisher:10828028 Chemical compound, drug Lipofectamine RNAiMAX Invitrogen/Thermo Fisher Invitrogen/Thermo Fisher:13778150 Chemical compound, drug Matrigel Corning Life Science Corning Life Science:354277 Chemical compound, drug MEM non-essential amino acids Gibco/Thermo Fisher Gibco/Thermo Fisher:11140050 Chemical compound, drug mTeSR1 medium STEMCELL Technologies STEMCELL Technologies:85850 Chemical compound, drug Opti-MEM reduced serum medium Thermo Fisher Thermo Fisher:31985070 Chemical compound, drug Penicillin/streptomycin Gibco/Thermo Fisher Gibco/Thermo Fisher:15140122 Chemical compound, drug Polybrene Sigma-Aldrich Sigma-Aldrich:107689 Chemical compound, drug Puromycin Sigma-Aldrich Sigma-Aldrich:P8833 Chemical compound, drug Recominant human basic fibroblast growth factor Promega Corporation Promega Corporation: G5071 Chemical compound, drug Sodium pyruvate Gibco/Thermo Fisher Gibco/Thermo Fisher:11360070 Chemical compound, drug Transferrin Sigma-Aldrich Sigma-Aldrich:T0665 Chemical compound, drug Y-27632 ROCK inhibitor Miltenyi Biotec Miltenyi Biotec: 130106538 Continued on next page Campbell et al. eLife 2018;7:e31023. .. DOI: https://doi.org/10.7554/eLife.31023 17 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Software, algorithm Code used for proteomics data analysis This paper (Jagannathan, 2017) The R code used for the proteomics data analysis can be accessed via github at https://github.com/sjaganna/ 2017-campbell_et_al Software, algorithm GraphPad Prism GraphPad Prism (https://graphpad.com) RRID:SCR_015807 Version 6 Software, algorithm ImageJ ImageJ (http://imagej.nih.gov/ij/) RRID:SCR_003070 Software, algorithm Proteome Discoverer Thermo Fisher RRID:SCR_014477 Version 1.4



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    Thermo Fisher f 10 nutrient mix gibco thermo fisher gibco thermo fisher
    F 10 Nutrient Mix Gibco Thermo Fisher Gibco Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/f+10+nutrient+mix+gibco+thermo+fisher+gibco+thermo+fisher/Dynabeads+Protein+G+for+Immunoprecipitation/10__7554_slash_elife__31023-218-94-97
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